Table 3 Strains and plasmids used in this study
Name | Description | Source |
|---|---|---|
Strains | ||
 M. neoaurum DSM 44704 | Wild type strain, Sterol consumer with no detectable intermediates |  |
 ΔkstD | kstD1, kstD2, and kstD3 deleted in M. neoaurum DSM 44704 | This study |
ΔkstDΔhsd4A | hsd4A deleted in ΔkstD strain | This study |
 ΔkstDΔfadA5 | fadA5 deleted in ΔKstD strain | This study |
 ΔkstDΔhsd4A-hsd4A | ΔkstDΔhsd4A strain harboring P38Mu-Hsd4A | This study |
 ΔkstDΔfadA5-fadA5 | ΔkstDΔfadA5 strain harboring P38Mu-FadA5 | This study |
 ΔkstDΔhsd4AΔfadA5 | hsd4A and fadA5 deleted in ΔkstD strain | This study |
 ΔkstDΔhsd4AΔfadA5-NK | ΔkstDΔhsd4AΔfadA5 strain harboring P38Mu-NK | This study |
Plasmids | ||
 PSBY1 | Derived from pMV261 and contains FnCpf1 C. glutamicum codon-optimized; KanR | [29] |
 PCR-Hyg | Plasmid for sgRNA production | [30] |
 Pam-KstD1 | PCR-Hyg containing kstD1 spacer | This study |
 Pam-KstD2 | PCR-Hyg containing kstD2 spacer | This study |
 Pam-KstD3 | PCR-Hyg containing kstD3 spacer | This study |
 Pam-Hsd4A | PCR-Hyg containing hsd4A spacer | This study |
 Pam-FadA5 | PCR-Hyg containing fadA5 spacer | This study |
 pMV306 | Mycobacterium integrative vector without promoter, kanR | [31] |
 P38Mu | pMV306 with Psmyc promoter, KanR | [32] |
 P38Mu-Hsd4A | Recombinant P38Mu for expression of hsd4A | This study |
 P38Mu-FadA5 | Recombinant P38Mu for expression of fadA5 | This study |
 P38Mu-NK | Recombinant P38Mu for expression of nox and katE | This study |