Fig. 3From: Production of 9,21-dihydroxy-20-methyl-pregna-4-en-3-one from phytosterols in Mycobacterium neoaurum by modifying multiple genes and improving the intracellular environment9-hydroxy steroids accumulation from 1 g L−1 phytosterols. a Time course of 9-OH-AD accumulation; b time course of 9-OH-4-HP accumulation; single deletion of hsd4A or fadA5 caused increment of 9-OH-4-HP, and double deletion of hsd4A and fadA5 could obviously increase the productivity and purity of 9-OH-4-HPBack to article page