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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Production of 9,21-dihydroxy-20-methyl-pregna-4-en-3-one from phytosterols in Mycobacterium neoaurum by modifying multiple genes and improving the intracellular environment

Fig. 2

Phenotypic analyses of the metabolites of phytosterol by M. neoaurum DSM 44074 and its derivative strains. a HPLC chromatogram comparison of the products of M. neoarum DSM 44704 and ΔkstD with 1 g L−1 phytosterols feed. b HPLC chromatogram comparison of the products of ΔkstD, ΔkstDΔhsd4A, ΔkstDΔfadA5 and ΔkstDΔhsd4AΔfadA5 with 1 g L−1 phytosterols feed. c HPLC chromatogram comparison of the products of ΔkstD, hsd4A complement strain ΔkstDΔhsd4A-hsd4A and fadA5 complement strain ΔkstDΔfadA5-fadA5, with 1 g L−1 phytosterols feed. d Structure of peak A, 9-OH-AD, and peak B, 9-OH-4-HP

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