Table 4 Oligonucleotides used in this study
Name | Target gene | Sequence (5′–3′) | Cohesive endsa |
|---|---|---|---|
Primer 1 | pta-ackA | ACGGCCAGTGGAATTCTGCGTGAGATGAAGTAAGGC | EcoRI |
Primer 2 | pta-ackA | GGTGTTGGTGAAAATGCCCA | |
Primer 3 | pta-ackA | ATTTTCACCAACACCACGTGTTTCCTACACCGATG | |
Primer 4 | pta-ackA | ATGATTACCCAAGCTTGTCCGTGTCGGATTTCATCA | HindIII |
Primer 5 | alaT | ACGGCCAGTGGAATTCCTAGTCCGTTTTCGACGATG | EcoRI |
Primer 6 | alaT | ACAGCACGTCCTTCATCTTC | |
Primer 7 | alaT | TGAAGGACGTGCTGTACCCCAACGTGTACGAAATC | |
Primer 8 | alaT | ATGATTACCCAAGCTTAAGTTTCAGGCATAGGCAGG | HindIII |
Primer 9 | avtA | ACGGCCAGTGGAATTCTCCATGAGGTCAAGCATGTC | EcoRI |
Primer 10 | avtA | CCGATGATTCAGAGGAATGG | |
Primer 11 | avtA | CCTCTGAATCATCGGAGAGCGATCTCTGCTTCTTC | |
Primer 12 | avtA | ATGATTACCCAAGCTTTTGATGGGGAGACTGTGGTT | HindIII |
Primer 13 | cat | ACGGCCAGTGGAATTCCGTAAAGCGGAGTTTTAGGC | EcoRI |
Primer 14 | cat | ATCTCTGAGTACGGTTACGC | |
Primer 15 | cat | ACCGTACTCAGAGATAACAGGTCGATTGCGTAGTC | |
Primer 16 | cat | ATGATTACCCAAGCTTAGCAACGTTGGTTACACCAG | HindIII |
Primer 17 | ldh | ACGGCCAGTGGAATTCTGGGTTAATTCGCCGGTGATCAG | EcoRI |
Primer 18 | ldh | GGTTGATCAGTGCAGTATGCGTATG | |
Primer 19 | ldh | CTGCACTGATCAACCCACTGCTCCACGGTGAATACG | |
Primer 20 | ldh | ATGATTACCCAAGCTTGGCAAGGTCCATGCTGACG | HindIII |
Primer 21 | mdh | ACGGCCAGTGGAATTCAATGACAACGGCGTGGCTTC | EcoRI |
Primer 22 | mdh | TCTGCGGCAATTCCTTCCAC | |
Primer 23 | mdh | AGGAATTGCCGCAGAGGGATCTCCAGAAGTTTCAG | |
Primer 24 | mdh | ATGATTACCCAAGCTTTTTCCATCAATAGCAGGCGC | HindIII |
Primer 25 | pqo | ACGGCCAGTGGAATTCTGGTCGCATCTCATCGATTG | EcoRI |
Primer 26 | pqo | GCATATCCTGGACCTGTACT | |
Primer 27 | pqo | CAGGATATGCTTTCCAGGACCACAAGAAGC | |
Primer 28 | pqo | ATGATTACCCAAGCTTCTTGCGCCTGCAAAGTTTCT | HindIII |