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Table 2 Strains and plasmids used in this study

From: Enhanced production of γ-amino acid 3-amino-4-hydroxybenzoic acid by recombinant Corynebacterium glutamicum under oxygen limitation

Name Relevant characteristics Reference or source
Strain   
Escherichia coli JM109 recA1 endA1 gyrA96 thi hsdR17(rK mK+) e14 (mcrA) supE44 relA1 Δ(lac-proAB)/F’ [traD36 proAB+ lacIq lacZΔM15] Takara Bio
Corynebacterium glutamicum ATCC 21799 l-Lysine producer resistant to S-2-aminoethyl-l-cysteine, a lysine analog ATCC
C. glutamicum strain KT01 C. glutamicum ATCC 21799 bearing pCACgriHI Kawaguchi et al. [11]
C. glutamicum strain HKC5021 Markerless mutant, Δldh (KaCgl_14550) of strain ATCC 21799 This study
C. glutamicum strain HKC5044 Markerless double mutant, ΔalaT ΔavtA (KaCgl_13940 and KaCgl_11440, respectively) of strain ATCC 21799 This study
C. glutamicum strain HKC5050 Markerless triple mutant, ΔpqoΔcat Δpta_ackA (KaCgl_11560, KaCgl_11130, KaCgl_13020 and KaCgl_13010, respectively) of strain ATCC 21799 This study
C. glutamicum strain HKC5053 Markerless mutant of Δmdh (KaCgl_08800) of strain ATCC 21799 This study
C. glutamicum strain HKC5037 HKC5021 bearing pCACgriHI This study
C. glutamicum strain HKC5051 HKC5044 bearing pCACgriHI This study
C. glutamicum strain HKC5052 HKC5050 bearing pCACgriHI This study
C. glutamicum strain HKC5057 HKC5053 bearing pCACgriHI This study
Plasmid   
pCACgriHI Cmr; E. coli–Corynebacterium sp. shuttle vector harboring griH and griI genes derived from Streptomyces griseus for 3,4-AHBA biosynthesis Kawaguchi et al. [11]
pK19mobsacB Kanr, mobilizable E. coli vector for the construction of insertion and deletion mutants of C. glutamicum (oriV, sacB, lacZα) ATCC
pK19mobsac-Δpta-ackAldh Kanr, pK19mobsacB with the deletion construct for pta-ackA (KaCgl_13010 and KaCgl_13020) of C. glutamicum ATCC 21799 This study
pK19mobsac-ΔalaT Kanr, pK19mobsacB with the deletion construct for alaT (KaCgl_13940) of C. glutamicum ATCC 21799 This study
pK19mobsac-ΔavtA Kanr, pK19mobsacB with the deletion construct for avtA (KaCgl_11440) of C. glutamicum ATCC 21799 This study
pK19mobsac-Δcat Kanr, pK19mobsacB with the deletion construct for cat (KaCgl_11130) of C. glutamicum ATCC 21799 This study
pK19mobsac-Δldh Kanr, pK19mobsacB with the deletion construct for ldh (KaCgl_14550) of C. glutamicum ATCC 21799 This study
pK19mobsac-Δmdh Kanr, pK19mobsacB with the deletion construct for mdh (KaCgl_08800) of C. glutamicum ATCC 21799 This study
pK19mobsac-Δpqo Kanr, pK19mobsacB with the deletion construct for pqo (KaCgl_29450) of C. glutamicum ATCC 21799 This study