Table 2 Strains and plasmids used in this study
Name | Relevant characteristics | Reference or source |
|---|---|---|
Strain | ||
Escherichia coli JM109 | recA1 endA1 gyrA96 thi hsdR17(rK– mK+) e14– (mcrA) supE44 relA1 Δ(lac-proAB)/F’ [traD36 proAB+ lacIq lacZΔM15] | Takara Bio |
Corynebacterium glutamicum ATCC 21799 | l-Lysine producer resistant to S-2-aminoethyl-l-cysteine, a lysine analog | ATCC |
C. glutamicum strain KT01 | C. glutamicum ATCC 21799 bearing pCACgriHI | Kawaguchi et al. [11] |
C. glutamicum strain HKC5021 | Markerless mutant, Δldh (KaCgl_14550) of strain ATCC 21799 | This study |
C. glutamicum strain HKC5044 | Markerless double mutant, ΔalaT ΔavtA (KaCgl_13940 and KaCgl_11440, respectively) of strain ATCC 21799 | This study |
C. glutamicum strain HKC5050 | Markerless triple mutant, ΔpqoΔcat Δpta_ackA (KaCgl_11560, KaCgl_11130, KaCgl_13020 and KaCgl_13010, respectively) of strain ATCC 21799 | This study |
C. glutamicum strain HKC5053 | Markerless mutant of Δmdh (KaCgl_08800) of strain ATCC 21799 | This study |
C. glutamicum strain HKC5037 | HKC5021 bearing pCACgriHI | This study |
C. glutamicum strain HKC5051 | HKC5044 bearing pCACgriHI | This study |
C. glutamicum strain HKC5052 | HKC5050 bearing pCACgriHI | This study |
C. glutamicum strain HKC5057 | HKC5053 bearing pCACgriHI | This study |
Plasmid | ||
pCACgriHI | Cmr; E. coli–Corynebacterium sp. shuttle vector harboring griH and griI genes derived from Streptomyces griseus for 3,4-AHBA biosynthesis | Kawaguchi et al. [11] |
pK19mobsacB | Kanr, mobilizable E. coli vector for the construction of insertion and deletion mutants of C. glutamicum (oriV, sacB, lacZα) | ATCC |
pK19mobsac-Δpta-ackAldh | Kanr, pK19mobsacB with the deletion construct for pta-ackA (KaCgl_13010 and KaCgl_13020) of C. glutamicum ATCC 21799 | This study |
pK19mobsac-ΔalaT | Kanr, pK19mobsacB with the deletion construct for alaT (KaCgl_13940) of C. glutamicum ATCC 21799 | This study |
pK19mobsac-ΔavtA | Kanr, pK19mobsacB with the deletion construct for avtA (KaCgl_11440) of C. glutamicum ATCC 21799 | This study |
pK19mobsac-Δcat | Kanr, pK19mobsacB with the deletion construct for cat (KaCgl_11130) of C. glutamicum ATCC 21799 | This study |
pK19mobsac-Δldh | Kanr, pK19mobsacB with the deletion construct for ldh (KaCgl_14550) of C. glutamicum ATCC 21799 | This study |
pK19mobsac-Δmdh | Kanr, pK19mobsacB with the deletion construct for mdh (KaCgl_08800) of C. glutamicum ATCC 21799 | This study |
pK19mobsac-Δpqo | Kanr, pK19mobsacB with the deletion construct for pqo (KaCgl_29450) of C. glutamicum ATCC 21799 | This study |