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Strain
| | |
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Escherichia coli JM109
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recA1 endA1 gyrA96 thi hsdR17(rK– mK+) e14– (mcrA) supE44 relA1 Δ(lac-proAB)/F’ [traD36 proAB+ lacIq lacZΔM15]
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Takara Bio
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Corynebacterium glutamicum ATCC 21799
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l-Lysine producer resistant to S-2-aminoethyl-l-cysteine, a lysine analog
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ATCC
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C. glutamicum strain KT01
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C. glutamicum ATCC 21799 bearing pCACgriHI
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Kawaguchi et al. [11]
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C. glutamicum strain HKC5021
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Markerless mutant, Δldh (KaCgl_14550) of strain ATCC 21799
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This study
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C. glutamicum strain HKC5044
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Markerless double mutant, ΔalaT ΔavtA (KaCgl_13940 and KaCgl_11440, respectively) of strain ATCC 21799
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This study
|
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C. glutamicum strain HKC5050
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Markerless triple mutant, ΔpqoΔcat Δpta_ackA (KaCgl_11560, KaCgl_11130, KaCgl_13020 and KaCgl_13010, respectively) of strain ATCC 21799
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This study
|
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C. glutamicum strain HKC5053
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Markerless mutant of Δmdh (KaCgl_08800) of strain ATCC 21799
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This study
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C. glutamicum strain HKC5037
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HKC5021 bearing pCACgriHI
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This study
|
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C. glutamicum strain HKC5051
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HKC5044 bearing pCACgriHI
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This study
|
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C. glutamicum strain HKC5052
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HKC5050 bearing pCACgriHI
|
This study
|
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C. glutamicum strain HKC5057
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HKC5053 bearing pCACgriHI
|
This study
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Plasmid
| | |
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pCACgriHI
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Cmr; E. coli–Corynebacterium sp. shuttle vector harboring griH and griI genes derived from Streptomyces griseus for 3,4-AHBA biosynthesis
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Kawaguchi et al. [11]
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pK19mobsacB
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Kanr, mobilizable E. coli vector for the construction of insertion and deletion mutants of C. glutamicum (oriV, sacB, lacZα)
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ATCC
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pK19mobsac-Δpta-ackAldh
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Kanr, pK19mobsacB with the deletion construct for pta-ackA (KaCgl_13010 and KaCgl_13020) of C. glutamicum ATCC 21799
|
This study
|
|
pK19mobsac-ΔalaT
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Kanr, pK19mobsacB with the deletion construct for alaT (KaCgl_13940) of C. glutamicum ATCC 21799
|
This study
|
|
pK19mobsac-ΔavtA
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Kanr, pK19mobsacB with the deletion construct for avtA (KaCgl_11440) of C. glutamicum ATCC 21799
|
This study
|
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pK19mobsac-Δcat
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Kanr, pK19mobsacB with the deletion construct for cat (KaCgl_11130) of C. glutamicum ATCC 21799
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This study
|
|
pK19mobsac-Δldh
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Kanr, pK19mobsacB with the deletion construct for ldh (KaCgl_14550) of C. glutamicum ATCC 21799
|
This study
|
|
pK19mobsac-Δmdh
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Kanr, pK19mobsacB with the deletion construct for mdh (KaCgl_08800) of C. glutamicum ATCC 21799
|
This study
|
|
pK19mobsac-Δpqo
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Kanr, pK19mobsacB with the deletion construct for pqo (KaCgl_29450) of C. glutamicum ATCC 21799
|
This study
|
