Fig. 3

Dissolved oxygen (DO)-stat cultivation for 3-amino-4-hydroxybenzoic acid (3,4-AHBA) production by recombinant C. glutamicum strain KT01. a The concentrations of glucose (squares), 3,4-AHBA (circles), b dry cell weight (DCW) (triangles), and DO concentration (continuous lines) are indicated. The DO-stat method was started after 24 h of cultivation, and two DO levels (0 [reds] and 2.6 ppm [blues]) were implemented and controlled by the speed of agitation. The data are presented as averages ± standard deviation calculated from the results of duplicate independent experiments