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Table 3 Characteristics of PhaC polymerases found in Cobetia and Halomonas

From: High natural PHA production from acetate in Cobetia sp. MC34 and Cobetia marina DSM 4741T and in silico analyses of the genus specific PhaC2 polymerase variant

Strain

PhaC2 MW (kDa)/pIN613/pIC-terminus

PhaC2 box

HC2 domain (E-value)

PhaC1 MW (kDa)/pI

PhaC1 box

Cobetia sp. MC34

105.4/4.63/10.34

SYCIG

8.77 × 10–9

–

–

Cobetia amphilecti KMM296

103.1/4.67/10.27

SYCIG

4.56 × 10–9

–

–

Halomonas sp. SF2003*

101.8/4.66/10.15

SYCIG

4.79 × 10–10

71.5/5.27

GYCLG

Halomonas sp. GDM18*

102.6/4.63/10.24

SYCIG

2.99 × 10–8

71.5/5.27

GYCLG

Cobetia sp. AM6

102.6/4.63/10.34

SYCIG

3.55 × 10–9

71.5/5.31

GYCLG

Cobetia sp. UCD-24C

103.9/4.76/10.19

SYCIG

2.24 × 10–8

–

–

Cobetia sp. QF1

95.1/4.77/10.23

SYCIG

3.79 × 10–11

–

–

Cobetia sp. ICG0124

106.1/4.74/10.23

SYCIG

4.32 × 10–12

40–50 kDa*

GYCLG

Cobetia sp. L2A1

99.9/4.85/10.06

SYCIG

1.86 × 10–7

65.6/5.43

GYCLG

Cobetia crustatorum SM1923

93.6/4.77/10.14

SYCIG

2.78 × 10–10

–

–

Cobetia crustatorum JO1

95.4/4.77/10.23

SYCIG

3.42 × 10–11

–

–

Cobetia marina DSM 4741T

106.4/4.74/10.35

SYCIG

1.77 × 10–10

64.0/5.31

GYCLG

Cobetia marina NBRC 15607

105.2/4.74/10.36

SYCIG

2.85 × 10–10

63.9/5.31

GYCLG

Cobetia marina T1

105.1/4.74/10.36

SYCIG

2.73 × 10–11

64.1/5.26

GYCLG

Cobetia sp. MM1IDA2H-1

105.0/4.74/10.40

SYCIG

3.39 × 10–7

–

–

Cobetia pacifica GPM2

105.9/4.74/10.38

SYCIG

1.14 × 10–9

–

–

Cobetia sp. IU 180733JP01

(5-11-6-3)

100.9/4.74/10.36

SYCIG

3.04 × 10–9

–

–

Cobetia sp. 190790JP01

(5-25-4-2)

106.2/4.74/10.38

SYCIG

3.09 × 10–9

–

–

Cobetia sp. MB87

100.4/4.66/10.22

SYCIG

2.91 × 10–10

–

–

Halomonas sp. R5-57

71.8/4.86/8.54**

SYCVG

–

66.7/5.29

GYCLG

Halomonas bluephagenesis

TD01

92.8/5.39/9.60***

GNCQAG

–

69.7/4.80

SYCVG

Halomonas sp. O-1

89.0/5.45/10.10***

GNCQAG

–

69.4/5.18

SYCVG

  1. PhaC1 and PhaC2 sequences found in Cobetia whole genome sequences from genome annotation and by BLAST search. Predicted Molecular weight (MW) is shown for the complete protein sequence while the isoelectric point (pI) is shown for the N-terminal 613 amino acids (pIN613) and for the extended C-terminus (pIC-terminus) for PhaC2 and for the whole protein sequence for PhaC1.Genome accession numbers are listed in Additional file 1: Table S1
  2. *Identified as belonging to Cobetia (this study)
  3. **The C-terminus consist of only 21 amino acids and thus non-homologous to Cobetia PhaC2
  4. ***Putative PhaC2, non-homologous to Cobetia PhaC2 (This study)