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Table 2 Genes encoding transporters, enzymes, transcriptional regulators and carbonosome proteins involved in PHA synthesis from acetate

From: High natural PHA production from acetate in Cobetia sp. MC34 and Cobetia marina DSM 4741T and in silico analyses of the genus specific PhaC2 polymerase variant

Gene description

Gene(s)

Cobetia sp. MC34

Cobetia marina DSM 4741T

Halomonas sp. SF2003

Acetyl-CoA transferase

(EC 2.3.1.9)

phaA (phaA/fadA)

+ [+]

IZU87_07675/(IZU87_02125)

++

BFX80_16410/(BFX80_14370)

++

C8233_08335/C8233_10360

Acetoacetyl-CoA reductase

(EC 1.1.1.36)

phaB

+

IZU87_12765

+

BFX80_01220

+

C8233_12530

Polyhydroxyalkanoate polymerase

(EC 2.3.1.-)

phaC1/phaC2

−/+

−/IZU87_04860

+/+

BFX80_07630/BFX80_12110

+/+

C8233_00780/C8233_06040

Phosphate acetyltransferase

(EC 2.3.1.8)

pta

+

IZU87_07815

+

BFX80_04540

+

C8233_15920

Bifunctional enoyl-CoA hydratase/phosphate acetyltransferase

bi-pta

–

 + 

BFX80_07620

 + 

C8233_00770

Acetate/propionate kinase

(EC 2.7.2.1)

ackA

–

 + 

BFX80_07615

 + 

C8233_00765

Acetate–CoA ligase

(EC 6.2.1.1)

acs

 + 

IZU87_06405

 + 

BFX80_02550

 + 

C8233_13850

Propionyl-CoA synthetase

(EC 6.2.1.17)

prpE

 + 

IZU87_15385*

 + 

BFX80_09760

 + 

C8233_03035

Citrate synthase

(EC 2.3.3.1)

gltA

 + 

IZU87_01890

 + 

BFX80_14605

 + 

C8233_08570

Aconitate hydratase

(EC 4.2.1.3)

acnA/acnB

+/+ 

IZU87_02425/IZU87_05790

+/+

BFX80_01545/BFX80_04820

+/+ 

C8233_12860/C8233_16190

Isocitrate lyase

(EC 4.1.3.1)

AceA

 + 

IZU87_14450

 + 

BFX80_03560

 + 

C8233_14950

Malate synthase

(EC 2.3.3.9)

AceB (glcB)

 + 

IZU87_07285

 + 

BFX80_16805

 + 

C8233_10755

Cation acetate symporter

–

 + 

IZU87_06430

 + 

BFX80_02575

 + 

C8233_13875

Phasin

phaP1/P2

+/+ 

IZU87_08295/IZU87_04855

+/+ 

BFX80_04055/BFX80_12115

+/+ 

C8233_15445/C8233_06045

Repressor

phaR

 + 

IZU87_03745

 + 

BFX80_15490

 + 

C8233_09450

3-hydroxybutyrate dehydrogenase

(EC 1.1.1.30)

bdhA

 + 

IZU87_16460

 + 

BFX80_07905

 + 

C8233_01125

Oxoacid transferases

(EC 2.8.3)

scoA/scoB

+/+ 

IZU87_09115/IZU87_09110

+/+ 

BFX80_11345/BFX80_11350

+/+ 

C8233_05175/C8233_05180

  1. The genes were identified from genome annotations [75, 76] and BLAST search against the different genomes
  2.  + indicates presence and number of gene(s), with locus tags, − indicate absence, [+] indicate ambiguity between the annotation methods Prokka [57] and NCBI’s Prokaryotic Annotation Pipeline [58]
  3. *Annotated as acetate–CoA ligase (EC 6.2.1.1) by NCBI’s Prokaryotic Annotation Pipeline [58], but as propionyl-CoA synthetase (EC 6.2.1.17) by KEGG [67]
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Microbial Cell Factories

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