Skip to main content

Table 1 Calculation of validation parameters for the screening protocol

From: Rapid screening of high expressing Escherichia coli colonies using a novel dicistronic-autoinducible system

Step

Average

SD

RSD (%)

LOD

LOQ

Fluorescence signal measurement (RFU)

34,791.44

 ± 753.31

2.16

3155.52

3497.40

SAK activity assay (IU/mg)

184.87

 ± 3.18

1.72

0.68

27.53

Number of double transformed clones

107.33

 ± 2.5

2.32

–

–

Obtaining a clone in each well of 96-microplate

1

 ± 0.00

0

–

–

  1. SD: standard deviation; RSD: relative standard deviation; LOD: limit of detection; LOQ: limit of quantification
Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us