Fig. 4

The LRE541 extract induces apoptosis in RKO and 7901 cell lines. a The apoptotic cells change in RKO and 7901 cell lines when treated with increasing concentrations of the LRE541 extract for 48 h by Annexin V and PI double-staining assay. b The quantification of necrotic cells, apoptotic cells and normal cells. The data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the control groups. c Early and late apoptotic cells of 7901 and RKO treated with varying concentrations of the LRE541 extract for 48 h