From: Genetic optimisation of bacteria-induced calcite precipitation in Bacillus subtilis
Strain | Relevant characteristicsa | Source/Constructionb |
---|---|---|
DH5α | Escherichia coli DH5α | Laboratory stock |
DSM 33 | Wild-type Sporosarcina pasteurii DSM 33 | DSMZ |
ATCC 9945a | Wild-type Bacillus paralicheniformis ATCC 9945a | ATCC |
W168 | Wild-type Bacillus subtilis W168 | Laboratory stock |
NCIB3610 | Wild-type Bacillus subtilis NCIB3610 | Laboratory stock |
HB13321 | W168 ytpB::MLSr | [58] |
WΔ (SGB666) | W168 ureABC::kanr | LFH: kanr amplified from pDG780 using primers SG0144/SG0145; upstream and downstream gene fragments amplified from W168 gDNA using primers SG0510/SG0511 and SG0512/SG0513. W168 transformed with PCR products joined using primers SG0510/SG0513. Correct gene deletion checked with primers SG0532/SG0147 and SG0533/SG0146 |
WΔ-SpU (SGB739) | W168 ureABC::kanr, lacA::PxylA-ureABCEFGDSp-MLSr | pBS2E-PxylA-ureABCEFGDSp → SGB666 |
W-SpU (SGB740) | W168 lacA::PxylA-ureABCEFGDSp-MLSr | pBS2E-PxylA-ureABCEFGDSp → W168 |
WΔ-U (SGB823) | W168 ureABC::kanr, amyE::PxylA-UTBp-catr | pBS1C-PxylA-UTBp → SGB666 |
WΔ-H (SGB824) | W168 ureABC::kanr, amyE::PxylA-ureHBp-cat | pBS1C-PxylA-ureHBp → SGB666 |
WΔ-U-H (SGB825) | W168 ureABC::kanr, amyE::PxylA-UTBp-ureHBp-cat | pBS1C-PxylA-UTBp-ureHBp → SGB666 |
WΔ-U-BpU (SGB826) | W168 ureABC::kanr, amyE::PxylA-UTBp-catr, thrC:: PxylA-ureABCEFGDBp-spcr | pXT-PxylA-ureABCEFGDBp → SGB823 |
WΔ-H-BpU (SGB827) | W168 ureABC::kanr, amyE::PxylA-ureHBp-catr, thrC:: PxylA-ureABCEFGDBp-spcr | pXT-PxylA-ureABCEFGDBp → SGB824 |
WΔ-U-H-BpU (SGB828) | W168 ureABC::kanr, amyE::PxylA-UTBp-ureHBp-catr, thrC:: PxylA-ureABCEFGDBp-spcr | pXT-PxylA-ureABCEFGDBp → SGB825 |
WΔ-BpU (SGB844) | W168 ureABC::kanr, thrC:: PxylA-ureABCEFGDBp-spcr | pXT-PxylA-ureABCEFGDBp → SGB666 |
NΔ (SGB905) | NCIB3610 ureABC::kanr | SPP1 transduction SGB666 → NCIB3610 |
NΔ-U (SGB912) | NCIB3610 ureABC::kanr, amyE::PxylA-UTBp-cat | SPP1 transduction SGB823 → SGB905 |
NΔ-H (SGB913) | NCIB3610 ureABC::kanr, amyE::PxylA-ureHBp-cat | SPP1 transduction SGB824 → SGB905 |
NΔ-U-H (SGB914) | NCIB3610 ureABC::kanr, amyE::PxylA-UTBp-ureHBp-cat | SPP1 transduction SGB825 → SGB905 |
NΔ-BpU (SGB915) | NCIB3610 ureABC::kanr, thrC:: PxylA-ureABCEFGDBp-spcr | SPP1 transduction SGB844 → SGB905 |
NΔ-U-BpU (SGB921) | NCIB3610 ureABC::kanr, amyE::PxylA-UTBp-cat, thrC:: PxylA-ureABCEFGDBp-spcr | SPP1 transduction SGB844 → SGB912 |
NΔ-H-BpU (SGB922) | NCIB3610 ureABC::kanr, amyE::PxylA-ureHBp-ca.r, thrC:: PxylA-ureABCEFGDBp-spcr | SPP1 transduction SGB844 → SGB913 |
NΔ-U-H-BpU (SGB923) | NCIB3610 ureABC::kanr, amyE::PxylA-UTBp-ureHBp-cat, thrC:: PxylA-ureABCEFGDBp-spcr | SPP1 transduction SGB844 → SGB914 |
W-Δd (SGB926) | W168 dltABCDE::zeor | LFH: zeor amplified from pBS1Z using primers SG0526/SG0527, upstream and downstream gene fragments amplified from W168 gDNA using primers SG0891/SG0892 and SG0893/SG0894. W168 transformed with PCR products joined using primers SG0891/SG0894. Correct gene deletion checked with primers SG0895/SG0527 and SG0526/SG0896 |
WΔ-Δd (SGB985) | W168 ureABC::kanr, ΔdltABCDE::zeor | gDNA SGB926 → SGB666 |
WΔ-Δd-H (SGB987) | W168 ureABC::kanr, amyE::PxylA-ureHBp-cat, dltABCDE::zeor | gDNA SGB926 → SGB824 |
WΔ-Δd-H-BpU (SGB988) | W168 ureABC::kanr, amyE::PxylA-ureHBp-ca.r, dltABCDE::zeor, thrC:: PxylA-ureABCEFGDBp-spcr | pXT-PxylA-ureABCEFGDBp → SGB987 |
WΔ-Δe-H (SGB992) | W168 ureABC::kanr, amyE::PxylA-ureHBp-cat, epsH::MLSr | LFH: MLS amplified from HB13321 using primers SG0634/SG0635, upstream and downstream gene fragments amplified from W168 gDNA using primers SG1009/SG1010 and SG1011/SG1012. SGB824 transformed with PCR products joined using primers SG1009/SG1012. Correct gene deletion checked with primers SG1021/SG0635 and SG0634/SG1022 |
WΔ-Δt-H (SGB993) | W168 ureABC::kanr, amyE::PxylA-ureHBp-cat, tasA::MLSr | LFH: MLS amplified from HB13321 using primers SG0634/SG0635, upstream and downstream gene fragments amplified from W168 gDNA using primers SG1013/SG1014 and SG1015/SG1016. SGB824 transformed with PCR products joined using primers SG1013/SG1016. Correct gene deletion checked with primers SG1023/SG0635 and SG0634/SG1024 |
WΔ-Δe-H-BpU (SGB995) | W168 ureABC::kanr, amyE::PxylA-ureHBp-cat, epsH::MLSr, thrC:: PxylA-ureABCEFGDBp-spcr | pXT-PxylA-ureABCEFGDBp → SGB992 |
WΔ-Δt-H-BpU (SGB996) | W168 ureABC::kanr, amyE::PxylA-ureHBp-cat, tasA::MLSr, thrC:: PxylA-ureABCEFGDBp-spcr | pXT-PxylA-ureABCEFGDBp → SGB993 |