Fig. 5

Contribution of biofilm components and surface charge to biomineralisation. A Crystal formation on LBGMC agar. Cells of the indicated strains were inoculated into the centre of biofilm-promoting agar plates containing urea and xylose and monitored over five days of incubation at 30 °C with images of the colony taken under a stereomicroscope at the time points indicated above. Scale bars represent 1 mm in size. The results shown are a representative series from three to four biological repeats. The images shown for WΔ-H-BpU are the same as shown in Fig. 4 and serve as the baseline control. B Quantification of crystal formation (crystal count per colony) over time. Individual data points for all biological repeats are shown, with the average value depicted as a line. The inset legend gives the p-values for differences by strain from a two-way ANOVA for each dataset compared to the WΔ-H-BpU parent strain. Δt, tasA deletion; Δe, epsH deletion; Δd, dltABCDE deletion; remaining strain nomenclature as in previous figures