Fig. 7From: OsnR is an autoregulatory negative transcription factor controlling redox-dependent stress responses in Corynebacterium glutamicumIn vivo assays showing the binding of the OsnR protein to the promoter region of the sigH gene. The binding activity was analyzed by measuring the β-galactosidase activity expressed from the PsigH::lacZ reporter plasmid (a) and the PsigB::lacZ reporter plasmid (b). The E. coli host contained a plasmid that expresses the OsnR protein and the reporter. A plasmid carrying only the reporter was used as a negative control (b). The cells were grown with DTT or diamide. Three independent experiments were performed, and the data represent three technical replicates from a representative experimentBack to article page