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Fig. 3 | Microbial Cell Factories

Fig. 3

From: OsnR is an autoregulatory negative transcription factor controlling redox-dependent stress responses in Corynebacterium glutamicum

Fig. 3

Binding of the purified OsnR protein to the promoter region of the osnR gene. a Schematic diagram of the chromosomal region comprising the osnR and adjacent genes. Numbers in circles indicate the DNA fragments used in the EMSA. b EMSA was performed using the purified His6-OsnR protein and the DNA fragments shown in a. The reactions in lanes 1, 2, 3, and 4 contained 0, 0.8, 1.0, and 1.2 μg of the OsnR protein, respectively. The arrow indicates the shifted band

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