Fig. 1

The mRNA levels of mycothiol biosynthetic genes in C. glutamicum cells and the sensitivities of the C. glutamicum mutants to alkylating agents and antibiotics. a C. glutamicum wild-type and osnR-overexpressing (P₁₈₀-osnR) cells were grown in the minimal medium, and the mRNA levels were measured using qRT-PCR as described in “Materials and methods” section. Error bars indicate the standard deviation of three replicates from a representative experiment. b Paper discs placed on MB plates containing lawns of C. glutamicum wild-type, osnR-deleted (ΔosnR), or P₁₈₀-osnR cells were spotted with iodoacetamide, N-ethylmaleimide, isoniazid, or ethionamide. Diameters of the zone of inhibition are shown in millimetres