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Fig. 5 | Microbial Cell Factories

Fig. 5

From: A synthetic promoter system for well-controlled protein expression with different carbon sources in Saccharomyces cerevisiae

Fig. 5

The synthetic promoter system significantly improved β-glucosidase secretion (a) and surface display (b). SED1, a gene encoding yeast cell wall protein which is a commonly used surface-displayed system. Enzyme activities were measured under glucose or galactose growth conditions for 12 h and 24 h, respectively. + represents in the presence of GAL1 or GAL80;—represents the deletion of GAL1 or GAL80.The enzyme activity was quantified by pNPG assay and the data are mean ± SD from three biological replicates

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