Fig. 1

UASs and their impact on GAL promoter strength in S. cerevisiae. a The architecture of the native GAL1 promoter. Upstream activating sequences (UAS_GAL1) and core promoter (cP_GAL1) are shown. U1, U2, U3, and U4 represented the four UASs of P_GAL1; M1 and M2 represent repressor Mig1p binding sites, which was responsible for glucose repression. b The sequence of UASs used in this study. U1-U4 and U4g are from ScP_GAL1 (S. cerevisiae, green), U5-U7 from SkP_GAL2 (S. kudriavzevii, purple) and U8 from ScP_GAL7 (S. cerevisiae, red). (c-d) The effect of UASs on the activities of synthetic promoters when cP_GAL1 (c) and cP_CYC1 (d) were used as core promoter. The activities of all synthetic promoters were tested after induction with 2% galactose for 24 h. Normalized fluorescence = Fluorescence intensity/OD₆₀₀. Data are mean ± SD (standard deviation) from three biological replicates