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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Production of soluble regulatory hydrogenase from Ralstonia eutropha in Escherichia coli using a fed-batch-based autoinduction system

Fig. 6

Glucose polymer boosting for soluble RH production. E. coli BQF8RH was cultivated in 50 mL EnPresso B medium with/without boosting in 250 mL UYF at 30 °C. Growth curves, specific and volumetric RH yield are presented. The RH yields were calculated from Coomassie stained gels after affinity purification and quantification with ImageJ as described in “Materials and methods”. A RH production using classic single-shot IPTG induction. B RH production using lactose-autoinduction. Black arrow: addition of booster and induction point; Grey arrow: addition of a second dose of booster

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