Fig. 3

RH production with autoinduction by varying concentrations of IPTG. E. coli BQF8RH was cultivated in 3 mL EnPresso B medium with/out booster on 24-deepwell plate at 30 °C, 250 rpm for 30 h. Autoinduction was achieved by addition of different IPTG concentrations as indicated and 0.5 g L⁻¹ glucose. Samples normalized to OD₆₀₀ of 5 were taken at the indicated time points and used for detection of HoxB levels by Western blotting (WB). A Growth curve, B WB for detection of HoxB yields, C specific HoxB (top) and volumetric HoxB yield (bottom). Both specific and volumetric yield were calculated relative to the HoxB yield reached after 30 h in non-boosted EnPresso induced with 0.5 g L⁻¹ lactose (indicated by ♦)