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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Production of soluble regulatory hydrogenase from Ralstonia eutropha in Escherichia coli using a fed-batch-based autoinduction system

Fig. 1

Effect of glucose and lactose on cell growth and HoxB production with autoinduction. E. coli BQF8RH was cultivated in 3 mL non-boosted EnPresso B medium on a 24-deepwell plate at 30 °C, 250 rpm for 24 h. Different concentrations of lactose and glucose were added as indicated to trigger RH production. A Final cell densities. B Samples normalized to OD600 of 5 were taken and HoxB detected by Western blotting. Specific HoxB yields were calculated relative to the HoxB yield reached in autoinduction with 1 g L−1 glucose and 2 g L−1 lactose (indicated by ♦)

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Microbial Cell Factories

ISSN: 1475-2859

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