Fig. 8

Summary of glycosylation efficiency (%) and glycoprotein titre (mg/L of glycosylated protein) of A scFv13R4, B scFv13R4CM, C RNase A, and D NGRP during production in glyco-competent E. coli K-12 by use of different cultivation conditions to modulate target protein folding. Glycoprotein titres were converted from the yield (mg/g DCW), cell growth, and total protein titre results given in the Figs. 5, 6, 7, and Tables 1, 2, 3, 4 and Additional file 2: Table S6 (“Methods” section). Colour symbols indicate the experiment or figure/table sources for the data (Fig; ¹ = Table 1, ² = Table 2, ³ = Table 3, ⁴ = Table 4, ⁵ = Additional file 2: Table S6). A similar wt and ΔdsbB cultivation were run in four different batches of experiment, which were (i) wt oxygen transfer experiment (10/50, Fig. 5A–D), (ii) wt against oxidoreductase knockout ΔdsbB (Fig. 6A–D), (iii) wt and ΔdsbB 0 cystine treatment (Fig. 7A–D), (iv) wt against oxidoreductase knockout ΔdsbC (Fig. 7E, F, Additional file 1: Figure S18A, B). A variation of inducer concentration for NGRP expression was used in the experiment of Fig. 6D (200 μM instead of 40 μM PPDA, “Methods” section)