Name | Sequence (5′ → 3′) | Usage |
---|---|---|
P_ackA01 | CGGTATCGGTGAAAACAGTGGTTTTAGAGCTAGAAATAGCAAGTT | Construction of pJOE_ackA sgRNA |
P_ackA02 | GAGAATTGAGTAAAATGTACCTACGCGGTATCGGTGAAAACAGTG | |
P_ackA03 | GGTCGACGGCCAACGAGGCCCCCCTCTCAACCGTGTTTCTATTTT | PCR of homology arms for ackA deletion |
P_ackA04 | TCAAGAGAATGTGCTTTCATGCGATGATTGACGCTCCTTTATACTCTGTA | |
P_ackA05 | TCAAGAGAATGTGCTTTCATGCGATGATTGACGCTCCTTTATACTCTGTA | |
P_ackA06 | ATGAGCGAAGGAATGGCCTTCGTGGCCAATAAGGCCTTTCTAG | |
P_ackA07 | CGACGGAAGTATCAAGACCTCCTGA | PCR of check ackA deletion |
P_ackA08 | ACTGGCAGATCACAAATGACCGTG | |
P_pta01 | GAATTGAACCTTACGCTGGGCGGGTTTTAGAGCTAGAAATAGCAAGTT | Construction of pJOE_pta spacer |
P_pta02 | GAGAATTGAGTAAAATGTACCTACGGAATTGAACCTTACGCTGGGCGG | |
P_pta03 | GGTCGACGGCCAACGAGGCCTGGATACAGCTTTTGCAGCT | PCR of homology arms for pta deletion |
P_pta04 | GAGCTGCCATTGTCTTCAATTTTAAATAAAACCTCCTCA | |
P_pta05 | TTGTCTTCAATTTTAAATAAAACCTCCTCAAAAAGTTACAA | |
P_pta06 | GGCCAAAAAGCTGATTTTATGGGCCAATAAGGCCTTTCTAG | |
P_pta07 | GTCCCGGAGCCTTTCTCTCT | PCR of check pta deletion |
P_pta08 | GAAGATCTGGCAACGACAAC | |
P_lctE01 | GATGTGATGGATTTAAACCAGTTTTAGAGCTAGAAATAGCAAGTT | Construction of pJOE_lctE spacer |
P_lctE02 | GAGAATTGAGTAAAATGTACCTACGGATGTGATGGATTTAAACCA | |
P_lctE03 | GGTCGACGGCCAACGAGGCCCTTTCAATGATCAACTGACGATTAC | PCR of homology arms for lactE deletion |
P_lctE04 | GAAACATACCCTGGAAGGATGATCCGCAACTTTAGAGTAAAGGG | |
P_lctE05 | GAAACATACCCTGGAAGGATGATCCGCAACTTTAGAGTAAAGGG | |
P_lctE06 | GAATCAAACAGACATGGCCCGGCCAATAAGGCCTTTCTAG | |
P_lctE07 | TGTAGCTGTGCTTTATCCTGATGATATTGC | PCR of check lactE deletion |
P_lctE08 | TGCTCGGGCCGGAATTAGCAAACATTTT | |
P_mmgA01 | CTGAATGAGGTGAAACCATGGTTTTAGAGCTAGAAATAGCAAGTT | Construction of pJOE_mmgA spacer |
P_mmgA02 | GAGAATTGAGTAAAATGTACCTACGCTGAATGAGGTGAAACCATG | |
P_mmgA03 | GGTCGACGGCCAACGAGGCCTCGGATGATTCATGCCCAGC | PCR of homology arms for mmgA deletion |
P_mmgA04 | TTCAAATCCCCTTTTTCATCATGTTGGTTTCACCTCATTCAGAAGATAGA | |
P_mmgA05 | TTCAAATCCCCTTTTTCATCATGTTGGTTTCACCTCATTCAGAAGATAGA | |
P_mmgA06 | TCCTTTAAGTGATGTCGTGCGATTAGGCCAATAAGGCCTTTCTAG | |
P_mmgA07 | GGCCTGAGTGAAGGACTTCCATAAT | PCR of check mmgA deletion |
P_mmgA08 | ATCAAGCTCTATCCCATCCGCTCCG |