Table 1 Composition of medium used in this study
Component | MM | BMM | BM | BMMY | LB |
|---|---|---|---|---|---|
Methanol | 0.5% | 0.5% | 1% | 0.5% | – |
YNB (Yeast Nitrogen Base) | 1.34% | 1.34% | – | 1.34% | – |
Biotin | 4 × 10−5% | 4 × 10−5% | – | 4 × 10−5% | – |
Potassium phosphate buffer, pH 6.0 | – | 100 mM | – | 100 mM | – |
Yeast extract | – | – | 0.5% | 1% | 0.5% |
Peptone | – | – | – | 2% | – |
Tryptone | – | – | – | – | 1% |
NaCl | – | – | – | – | 1% |
5 N NaOH | – | – | – | – | pH 7.0 |