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Table 1 Composition of medium used in this study

From: Establishment of a co-culture system using Escherichia coli and Pichia pastoris (Komagataella phaffii) for valuable alkaloid production

Component MM BMM BM BMMY LB
Methanol 0.5% 0.5% 1% 0.5%
YNB (Yeast Nitrogen Base) 1.34% 1.34% 1.34%
Biotin 4 × 10−5% 4 × 10−5% 4 × 10−5%
Potassium phosphate buffer, pH 6.0 100 mM 100 mM
Yeast extract 0.5% 1% 0.5%
Peptone 2%
Tryptone 1%
NaCl 1%
5 N NaOH pH 7.0
  1. MM: minimal methanol; BMM: buffered minimal methanol; BMMY: buffered methanol-complex medium