Fig. 3

Growth of E. coli and (S)-reticuline production from glycerol. Growth was evaluated by measuring the optical density at 600 nm (a), and the time-dependent production of (S)-reticuline in E. coli cells (b) and the medium (c) was determined. E. coli cells were cultured in LB, LB containing MeOH (0.5%) or the BMMY medium. These media contained IPTG (0.1 mM), glycerol (5 g/L), and appropriate antibiotics. Cells and media were sampled at the times indicated. Results indicate mean ± standard deviation of triplicate experiments