Fig. 2

Growth of P. pastoris and (S)-stylopine production from (S)-reticuline in various media. Growth was evaluated by measuring the optical density at 600 nm (a), and the time-dependent production of (S)-stylopine in P. pastoris cells (b) and the medium (c) was determined. P. pastoris cells were cultured in each medium containing 100 µM (S)-reticuline, and sampled at the times indicated. Results indicate mean ± standard deviation of triplicate experiments