Table 2 Information about 4 genes cloned from the genome of H. camelliae WT00C and the primers used in PCR
Gene | Primers | DNA sequence of the primer | Gene size (bp) | Gene position at the genomic DNA | Protein name annotated in NCBI database |
|---|---|---|---|---|---|
1 | Tan1-F Tan1-R | 5’-GGAATTCCATATGACGTCTTCTTTCATCTGGA-3’ 5’-CCGCTCGAGGCCCAGCAGGAACTGG-3’ | 900 | 3778213–3779112 | alpha/beta hydrolase |
2 | Tan2-F Tan2-R | 5’-GGAATTCCATATGACGAAGCTACCCGATAAC-3’ 5’-CCGCTCGAGCGCCCCCTTGTAAGGG-3’ | 1458 | 3719942–3721399 | GGDEF,domain-containing protein |
3 | Tan3-F Tan3-R | 5’-GGAATTCCATATGCAGGGATCGGCG-3’ 5’-CCGCTCGAGTTCGGCCAGGCTGAC-3’ | 450 | 5544953–5545402 | acyl-CoA dehydrogenase family protein |
4 | Tan4-F Tan4-R | 5’-GGAATTCCATATGGTCGATCTGTC-3’ 5’-CCGCTCGAGACGTGCCGGACGGGT-3’ | 1107 | 4574529–4575635 | prolyl oligopeptidase family serine peptidase/ alpha/beta hydrolase |