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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Heterologous production of novel and rare C30-carotenoids using Planococcus carotenoid biosynthesis genes

Fig. 3

Functional analysis of orfs. A HPLC chromatogram of cell extracts of the E. coli expressing pAC-HIO3. B HPLC chromatograms of the extracts of the E. coli expressing the plasmids pAC-HIMO1 (upper), pAC-HIMO2 (middle), and pAC-HIMO4 (lower). C HPLC chromatograms of the extracts of the E. coli expressing the plasmids pAC-HIMNO2 (upper), pAC-HIMNO4 (middle), and pAC-HIMNO7 (lower). D HPLC chromatograms of the extracts of the E. coli expressing the plasmids pAC-HIMNFO2 (upper) and pAC-HIMNFO4 (lower). E HPLC chromatogram of the extracts of the E. coli expressing the plasmid pAC-HIMNFNbO5. F The spectra correspond to the peak 1: 4,4′-diapophytoene, peak 2: 4,4′-diaponeurosporene, peak 3: 5-hydroxy-5,6-dihydro-4,4′-diaponeurosporene, peak 4: 5-hydroxy-5,6-dihydro-4,4′-diapolycopene, peak 5: 5-glucosyl-5,6-dihydro-4,4′-diapolycopene. Inset show the bacterial cell pelette of each recombinant E. coli

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Microbial Cell Factories

ISSN: 1475-2859

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