Table 3 Plasmids used in this study
From: Multivalent poultry vaccine development using Protein Glycan Coupling Technology
Plasmid | Description | Antibiotic resistance(s) | Reference or Source |
|---|---|---|---|
pCVD442 | Suicide donor plasmid based on R6K replicon and sacB sucrose counter selection marker | Amp | [49] |
pKD4 | Template plasmid for amplification of kanamycin cassette flanked with FRT sites | Kan | [80] |
pEC415 | Plasmid for L-arabinose inducible expression, Para | Amp | [81] |
pEXT20 | Plasmid for IPTG inducible expression, Ptac | Amp | [62] |
pFPV25.1 | Plasmid for constitutive expression, PRpsm | Amp | [63] |
pACYCpgl | Plasmid encoding C. jejuni pgl locus, constitutive expression | Cm | [24] |
pACYCpglΔpglB | Plasmid encoding C. jejuni pgl locus depleted of OST PglB, constitutive expression | Cm, Kan | [46] |
pEC415-G-ExoA(2) | L-arabinose inducible expression of His-tagged Exotoxoid A with 2 PglB glycosylation sequons under DsbA periplsmic signal | Amp | [82] |
pEC415-G-ExoA(10) | L-arabinose inducible expression of His-tagged Exotoxoid A with 10 PglB glycosylation sequons under DsbA periplsmic signal | Amp | [56] |
pSECpgl | Suicide plasmid for genomic integration of C. jejuni pgl locus in APEC Χ7122 genome | Amp, Kan | This study |
pEXT20-G-NetB(2) | IPTG inducible expression of His-tagged NetB toxoid with 2 PglB glycosylation sequons under PelB periplasmic signal | Amp | This study |
pEXT20-G-NetB(10) | IPTG inducible expression of His-tagged NetB toxoid with 10 PglB glycosylation sequons under PelB periplasmic signal | Amp | This study |
pEXT20-unG-NetB(10) | IPTG inducible expression of His-tagged NetB toxoid with 10 non-functional PglB glycosylation sequons (NQ) under PelB periplasmic signal | Amp | This study |
pFPV25.1-G-NetB(10) | Constitutive expression of His-tagged NetB toxoid with 10 PglB glycosylation sequons under PelB periplasmic signal | Amp | This study |
pFPV25.1-unG-NetB(10) | Constitutive expression of His-tagged NetB toxoid with 10 non-functional PglB glycosylation sequons (NQ) under PelB periplasmic signal | Amp | This study |