Fig. 2
From: Multivalent poultry vaccine development using Protein Glycan Coupling Technology
Generation of χ7122 pgl integrants. a Colony PCR confirmation of correct integration of the pgl locus into APEC χ7122 genome. Ten NalR, KanR, sucroseR, AmpS colonies were screened (lanes 1–10), χ7122 wt (lanes 11–12) was used as a negative control. Diagnostic junction amplicons 1 and 2 (~ 1250 bp) confirm all 10 clones screened are correct. b Volcano plot displaying differentially expressed genes comparing χ7122 pgl to wt χ7122. Positive x axis represents up-regulation in χ7122 pgl. Negative x axis represents up-regulation in wt χ7122. Red points have adjusted p-value < 0.05 0.04% of reads from χ7122 pgl libraries align to the C. jejuni pgl locus confirming its expression at the transcriptional level. Libraries were prepared in triplicate of PCR-positive clone 2