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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Regulating the T7 RNA polymerase expression in E. coli BL21 (DE3) to provide more host options for recombinant protein production

Fig. 3

Expression of three membrane proteins in three engineered strains and control strain BL21 (DE3). A Fluorescence intensity of Codb-EGFP of BL21 (DE3), BL21 (DE3::ara), BL21 (DE3::rha) and BL21 (DE3::tet) in the whole fermentation process and the percentage of plasmid-carrying cells in B. C Fluorescence intensity of Ecb-EGFP of BL21 (DE3), BL21 (DE3::ara), BL21 (DE3::rha), and BL21 (DE3::tet) in the whole fermentation process and the percentage of plasmid-carrying cells in D. E Fluorescence intensity of Yidc-EGFP of BL21 (DE3), BL21 (DE3::ara), BL21 (DE3::rha), and BL21 (DE3::tet) in the whole fermentation process and the percentage of plasmid-carrying cells in F. Values and error bars represent the means and the deviations from triplicate experiments. CodB, E. coli cytosine transporter protein. Ecb, E. coli F-ATPase subunit b. YidC, E. coli membrane protein insertase/foldase

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Microbial Cell Factories

ISSN: 1475-2859

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