Table 2 List of bacterial strains and plasmids used in this study

Strains

 Escherichia coli

  JM109

mcrA recA1 supE44 endA1 hsdR17

[40]

(r_K⁻m_K⁺) gyrA96 relA1 thi Δ(lac-proAB)

F′[traD36 proAB⁺ lacI^q lacZ ΔM15]

 Bacillus subtilis

  JABs24

trp+ ΔmanPA sfp⁺

[9]

  JABs32

ΔmanPA sfp⁺ spo0A3 abrB*

[15]

  KM1053

ΔmanPA sfp⁺ spo0A3 abrB*

This study

amyE::[P_srfA-lacZ, spcR]

  KM1016

168 trp⁺ ΔmanPA sfp⁺

[12]

amyE::[P_srfA-lacZ, spcR]

  KM1019

168 trp⁺ ΔmanPA sfp⁺ ΔabrB::loxP

This study

amyE::[P_srfA-lacZ, spcR]

  KM1043

168 trp⁺ ΔmanPA sfp⁺ abrB::abrB*-cat

This study

amyE::[P_srfA-lacZ, spcR]

  KM1018

168 trp⁺ ΔmanPA sfp⁺

This study

Δspo0A::loxP-ermC-loxP

amyE::[P_srfA-lacZ, spcR]

  KM1020

168 trp⁺ ΔmanPA sfp⁺

This study

Δspo0A::loxP-ermC-loxP ΔabrB::loxP

amyE::[P_srfA-lacZ, spcR]

KM1036

168 trp⁺ ΔmanPA sfp⁺

This study

Δspo0A::loxP-ermC-loxP abrB::abrB*-cat

amyE::[P_srfA-lacZ, spcR]

KM1028

168 trp⁺ ΔmanPA sfp⁺ Δabh::loxP

This study

amyE::[P_srfA-lacZ, spcR]

  KM1029

168 trp⁺ ΔmanPA sfp⁺

This study

Δspo0A::loxP-ermC-loxP Δabh::loxP

amyE::[P_srfA-lacZ, spcR]

  KM681

168 trp⁺ ΔmanPA sfp⁺

This study

Δspo0A::loxP-ermC-loxP abrB::abrB*-cat

  BKE24220

trpC2 spo0A::erm

Bacillus Genetic Stock Center

  BKE14480

trpC2 abh::erm

Bacillus Genetic Stock Center

  BKE00370

trpC2 abrB::erm

Bacillus Genetic Stock Center

Plasmids

 pKAM446

ori_pUC18, bla, rop, ermC,

[12]

amyE′-[ter-P_srfAA-lacZ, spcR]-′amyE

 pJOE7644.2

ori_pUC18, bla, P_manP-manP, spcR, ′manR-ctaO′

[21]