Fig. 5

Establishing anaerobicity in 96-well microplates. a Effectiveness of various seals in preventing oxygen penetration into microplates containing E. coli MG1655 in RDM, sealed within an anaerobic chamber. The biomass within each microplate are represented as violin plots. To the right of each violin plot, the distribution of biomass yields are represented as heatmaps showing deviation of the biomass yields from the median biomass yield within that plate. b Time-course showing cell density and instantaneous growth rate of E. coli MG1655 in RDM with and without a layer of oil in the presence of oxygen and with a layer of mineral oil inside an anaerobic chamber