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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Automation assisted anaerobic phenotyping for metabolic engineering

Fig. 4

Automated photometric pipette calibration. a Change in pipetting error due to an increase in the air gap. b Workflow for automated photometric calibration. The liquid handler is made to pipette a photometric standard at different levels onto a microplate. The absorbance data of the microplate are recorded and fed to a pythonic script which automatically calculates pipetting errors and calibration parameters for the pipette. c Pre and post-calibration pipetting error with the air-gap adjusted to ensure sterility. The maximum allowable error was obtained from ISO8655 standards. Accuracy ranges for manual pipettes were obtained from various manufacturers of multi-channel pipettes

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