Table 2 Pinene production in D. radiodurans after 72Â h of shake flask fermentation
From: Metabolic engineering of Deinococcus radiodurans for pinene production from glycerol
Host | Plasmid-expressed proteins | Titer [mg/L] | Productivity [ug pinene/h] | Yield [mg pinene/g glycerol] | Medium |
|---|---|---|---|---|---|
WT | PS-IspA | 0.5 ± 0.1 | 7 | – | Rich Medium |
|  | PS-IspA-DXS | 0.7 ± 0.1 | 10 | – | Rich Medium |
∆dr1395 | PS-IspA | 1.4 ± 0.2 | 19 | – | Rich Medium |
|  | PS-IspA-DXS | 1.8 ± 0.2 | 25 | – | Rich Medium |
∆dr0862 | PS-IspA | 2.1 ± 0.1 | 29 | – | Rich Medium |
|  | PS-DXS | 0.6 ± 0.1 | 8 | – | Rich Medium |
|  | PS:IspA-DXS | 1.7 ± 0.1 | 24 | – | Rich Medium |
|  | IspA:PS-DXS | 2.4 ± 0.2 | 33 | – | Rich Medium |
|  | PS-IspA-DXS | 2.6 ± 0.2 | 36 | – | Rich Medium |
|  | PS-IspA-DXS | 3.2 ± 0.2 | 45 | 0.13 | Minimal Glycerol Medium |
E. coli EcpsB | MEV-PS | 0.9 ± 0.2 | 13 | – | Rich Medium |
E. coli EcpsB | MEV-PS | 0.65 ± 0.1 | 9 | 0.026 | Minimal Glycerol Medium |