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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Metabolic engineering of Deinococcus radiodurans for pinene production from glycerol

Fig. 2

Schematic representation of the pinene production strategy and modifications to enhance the production in D. radiodurans. D. radiodurans carbohydrate sources including glycerol, glucose, and amino acids catabolism and pyruvate (Pyr) production in the cell. Pyr with glyceraldehyde 3-phosphate (GA3P) is converted to 1-Deoxy-D-Xylulose 5-Phosphate (DXP) by the enzyme 1-deoxy-d-xylulose-5-phosphate synthase (DXS). Following this pathway, isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) molecules are produced. Afterward, GPP synthase produces a GPP molecule from these two molecules. Finally, pinene synthase (Ps) catalyzes synthesis of pinene by cyclizing GPP. Black arrows indicate the natural path inside the cell; purple arrows indicate overexpression in the pathway; green arrows indicate heterologous expression, and the cross sign indicates the inactivation of the gene in the cell. The abbreviations of the pathway are as follows: Ec. GPPs, E. coli GPP synthase gene; Dr1395, native GPP synthase; Dr0862, native phytoene synthase

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