Fig. 5From: Synthesis of (−)−deoxypodophyllotoxin and (−)−epipodophyllotoxin via a multi-enzyme cascade in E. coliLC/MS analysis (method 2) of CYP82D61 activity for (−)−deoxypodophyllotoxin 6 hydroxylation in comparison to the respective negative control. Reaction conditions: 25 °C, 1,500 rpm (Eppendorf ThermoMixer C), 2 ml reaction tubes with open lids, 500 µL reaction volumeBack to article page