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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Synthesis of (−)−deoxypodophyllotoxin and (−)−epipodophyllotoxin via a multi-enzyme cascade in E. coli

Fig. 4

 A Schematic picture of the sequential two-cell biotransformation of (−)−matairesinol 1 to (−)−deoxypodophyllotoxin 6. After 3 h conversion by module one, module two was added to the reaction. B LC/MS analysis (method 4) at various time points of the reaction. Reaction conditions: 25 °C, 250 rpm (Orbital shaker), baffled Erlenmeyer flasks, 2 × 10 mL cell suspension, 200 µM (−)−matairesinol 1

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