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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Synthesis of (−)−deoxypodophyllotoxin and (−)−epipodophyllotoxin via a multi-enzyme cascade in E. coli

Fig. 2

LC/MS analysis to monitor activity of E. coli whole cell biocatalysts expressing 2-ODD. A (−)−yatein 5 depletion followed over 30 min (analysis method 3). B 3 h conversion of (−)−yatein 5 (analysis method 1). Reaction conditions: 25 °C, 1,500 rpm (Eppendorf ThermoMixer C), 2 ml reaction tubes with open lids, 500 µl cell suspension (70 g/L in resuspension buffer), 200 µM (−)−yatein 5

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Microbial Cell Factories

ISSN: 1475-2859

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