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Table 1 Plasmids used in this study

From: Scaling production of GFP1-10 detector protein in E. coli for secretion screening by split GFP assay

Name

Description

Resource

pET22b-sfGFP1-10

pET22b(+) with sfgfp1-10 gene under control of PT7

[13]

pPBEx2-NprE-Cutinase-GFP11

F. solani f. sp.  pisi cutinase gene with N-terminal B. subtilis signal peptide NprE and C-terminal GFP11-tag cloned into pPBEx2 [20] via PstI and SacI, Ptac

This study

pPBEx2-Pel-Cutinase-GFP11

F. solani f. sp.  pisi cutinase gene with N-terminal B. subtilis signal peptide Pel and C-terminal GFP11-tag cloned into pPBEx2 [20] via PstI and SacI, Ptac

This study