Fig. 6From: Scaling production of GFP1-10 detector protein in E. coli for secretion screening by split GFP assayImpact of incubation temperature on split GFP assay. Fluorescence was measured after 16 h of incubation at different temperatures without shaking. Bars indicate mean value of eight replicates with standard deviation. Identical detector solution was mixed with supernatant from C. glutamicum pPBEx2-NprE-Cutinase-GFP11 cultivation in shake flask with 6 h of cutinase-GFP11 expressionBack to article page