Fig. 3
From: Scaling production of GFP1-10 detector protein in E. coli for secretion screening by split GFP assay
Split GFP assay with GFP1-10 from fed-batch fermentation. Cell samples were taken after 23 h of fed-batch fermentation with IPTG or lactose as inducer. Optical densities were adjusted to 20 and cells were disrupted by French press. GFP1-10 was purified from the inclusion body fraction and used for detection of cutinase-GFP11 in C. glutamicum pPBEx2-NprE-Cutinase-GFP11 supernatant by split GFP assay at 20 \(^\circ\)C