Fig. 2
From: Scaling production of GFP1-10 detector protein in E. coli for secretion screening by split GFP assay
Fed-batch fermentation for production of GFP1-10. On-off settings for constant glucose feeding were triggered by dissolved oxygen signal. GFP1-10 expression in E. coli BL21(DE3) pET22b-sfGFP1-10 was induced by constant feeding of IPTG (A–C) or lactose (D–F) starting in parallel with glucose feeding until calculated inducer concentrations of 1 mM IPGT or 10 g/l lactose were reached in the bioreactor. Dissolved oxygen and glucose feed were measured for two biological replicates during fed-batch fermentation, respectively. Samples were taken for cell dry weight measurement and HPLC analysis of sugars. Cell samples were harvested after 23 h for purification of GFP1-10 detector protein