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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Scaling production of GFP1-10 detector protein in E. coli for secretion screening by split GFP assay

Fig. 1

Batch production of GFP1-10 in shake flasks and laboratory-scale bioreactors. A Optical density at 580 nm during cultivation of E. coli BL21(DE3) pET22b-sfGFP1-10 in shake flask or stirred tank reactor (STR) with LB or DeLisa medium. Dotted lines indicate induction with 200 \(\upmu\)M IPTG. B Oxygen transfer rate during batch fermentation from two biological replicates in LB and DeLisa medium. Dotted lines indicate induction of biological replicates with 200 \(\upmu\)M IPTG. C Microscopic images taken during cultivation at different time points from shake flask with LB (1–3) and bioreactors with LB (4–6) or with DeLisa medium (7–9). Inclusion bodies are the bright spots mainly located at the cell poles. Due to the good comparability of the biological replicates from bioreactors and for a better overview, only the optical densities and microscope images of STR 1 are shown

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