Table 2 Apparent catalytic efficiencies (k_cat/K_m, mM⁻¹ min⁻¹) for FFCA oxidation by HMFO WT and variants, at different pH values

0.24 ± 0.04

1.6 ± 0.6

0.21 ± 0.01

8.2 ± 2.2

24.9 ± 4.3

0.18 ± 0.01

3. 0 ± 0.2

0.55 ± 0.10

13.6 ± 1.4

51.6 ± 11.1

0.10 ± 0.01

4.9 ± 1.7

1.84 ± 0.32

42.3 ± 14.4

76.8 ± 11.2

0.033 ± 0.001

4.1 ± 0.7

1.65 ± 0.24

43.2 ± 2.6

95.9 ± 12.2

0.023 ± 0.001

3.7 ± 0.3

1.84 ± 0.32

66.4 ± 7.3

130.0 ± 16.0

0.009 ± 0.001

3.2 ± 0.3

1.65 ± 0.24

44.8 ± 5.2

45.1 ± 2.9