Fig. 3From: Optimizing operational parameters for the enzymatic production of furandicarboxylic acid building blockComparison of H2O2 and FDCA production during FFCA oxidation by HMFO. Reactions were performed at 28 °C in 50 mM Tris/HCl, pH 7.5, between FFCA (1.5 mM) and HMFO WT (2.5 µM). The products, FDCA and H2O2, were quantified after separation of the reaction mixture by HPLC and with AmplexRed/HRP assay, respectively. Mean and standard deviation values are shownBack to article page