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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Overproducing the BAM complex improves secretion of difficult-to-secrete recombinant autotransporter chimeras

Fig. 3

Co-overexpression of BAM improved the secretion of HbpD-Calm or HbpD-GFPnb chimeras. A Schematic representation and passenger models for HbpD-SpT2-Calm and HbpD-GFPnb. Coupling of SpC2-mScarlet allows detection of surface displayed HbpD-Spt2-Calm fusions, while binding of GFP to the GFPnb allows the detection of surface displayed HbpD-GFPnb. B, C Coomassie-stained SDS-PAGE gels with whole cell lysates of E. coli BL21 (DE3) cultures expressing HbpD chimeras. B Expression of HbpD-SpT2-Calm with and without overexpression of BAM compared to HbpD-SpT2. C Expression of HbpD-GFPnb with and without overexpression of BAM complex compared to HbpD-SpT2. D, E Fold change of fluorescence as detected by flow cytometry for binding of SpC2-mScarlet (D) or GFP (E) to HbpD-SpT2-Calm and HbpD-GFPnb when expressed with or without overproduced BAM complex. ***p < 0.005

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