Fig. 1

Sequence, localization and phylogenetic analysis of the l-arabinose transporter Trire2_104072. a Difference between exons in QM6a and RUT-C30 genome annotations of Trire2_104072 b Amino acid sequence of Trire2_104072 colored according to localization predicted by TMHMM (red = intracellular, blue = membrane, purple = membrane with lower probability, green = extracellular). The arrow denotes the start codon at the QM6a genome annotation and the asterisks denote the three predicted medium confidence ubiquitination sites. The proline at position 388 which is mutated in XLT1 is marked with a rectangle c Plot of the predicted membrane localization. Line shows the predicted membrane localization probability calculated by TMHMM, while the colored segments show transmembrane domains predicted by Phobius. Numbers of the TMDs are indicated on top of the segments. d Maximum-likelihood tree of Trire2_104072 with other published transporters capable of transporting l-arabinose and l-arabitol (AmLat2). The scale bar indicates number of substitutions per site, and the symbols at the internal nodes present bootstrap support values from 100 runs. The presence of sequence determinants discussed in text is indicated by shaded box. Am, Ambrosiozyma monospora; Nc, Neurospora crassa; Sc, Saccharomyces cerevisiae; At, Arabidopsis thaliana; Pg, Pichia guilliermondii; Ss, Scheffersomyces stipitis; Km, Kluyveromyces marxianus; Mt, Myceliophtora thermophila; Pc, Penicillium chrysogenum