Fig. 6

Cytoplasmic distribution of GUS11 in B. subtilis cells detected in vivo by iSplit GFP assay. Microfluidic chambers were inoculated with B. subtilis DB430 harboring pHT01 and pBSMul1 empty vector A or pHT01-sfGFP and pBSMul1 B as controls. Bacilli harboring pHT01-iSplitGFP and one of the gus11 expression vectors, pBS-4nt-GUS11 C or pBS-8nt-GUS11 D, respectively. Overall growth was analyzed by phase contrast microscopy. GUS11 formation and sfGFP production were detected with a GFP filter whereas dead cells were detected by using propidium iodide and an mCherry filter. All three channels were merged. For better visibility, the image was zoomed in at representative points. Cells were cultivated in optimized LB medium with 1 mM IPTG and 1 µM propidium iodide at 30 °C for approximately 18 h