Fig. 3
From: The iSplit GFP assay detects intracellular recombinant proteins in Bacillus subtilis
Gradual production of GUS11 determined as enzymatic activity and iSplit GFP assays. B. subtilis DB430 was transformed with pBS-Xnt-GUS11 plasmids coding for GUS11 and harboring the strong constitutive promoter PHpaII and ribosome binding site spacers of variable length (4 to 12 nucleotides; indicated by Xnt in plasmid name) and with the GFP1-10(TGA11) detector encoded on plasmid pHT01-iSplitGFP harboring the IPTG inducible promoter Pgrac. A Schematic presentation of plasmid construct. Parts of DNA are not drawn to scale; B relative enzymatic activity and iSplit GFP fluorescence of GUS11 were measured in biological and technical triplicates. Error bars indicate the respective standard deviation. The expression of detector protein was induced by addition of 1 mM IPTG