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Fig. 2 | Microbial Cell Factories

Fig. 2

From: The iSplit GFP assay detects intracellular recombinant proteins in Bacillus subtilis

Fig. 2

Analysis of protein and transcript accumulation for sfGFP and the detector variants GFP1-10 and GFP1-10(TGA11). A Schematic presentation of the gene variants encoding either sfGFP of the detector. B Analysis of expressed proteins by SDS-PAGE and subsequent staining with Coomassie Brilliant Blue G-250; M: PageRuler™ Prestained Protein Ladder (Thermo Fisher Scientific) was used as a marker. C Change of transcript amounts were determined with RT-qPCR. All samples were obtained by cultivating B. subtilis DB430 harboring the sfGFP-encoding vector pHT01-sfGFP or one of the detector plasmids pHT01-GFP1-10 or pHT01-iSplitGFP in biological and technical triplicates. Error bars indicate the respective standard deviation. Expression was induced by addition of 1 mM IPTG

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